抗孕激素RU486可防止大鼠体内促黄体生成素（LH）依赖性抑制素血清浓度的降低。

Antiprogestagen RU486 prevents the LH-dependent decrease in the serum concentrations of inhibin in the rat.

作者信息

Tébar M, Bellido C, Uilenbroek J T, Sánchez-Criado J E

机构信息

Department of Physiology, Faculty of Medicine, University of Córdoba, Spain.

出版信息

Cell Mol Neurobiol. 1996 Jun;16(3):283-95. doi: 10.1007/BF02088096.

DOI:10.1007/BF02088096

PMID:8818397

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11563109/

Abstract

In the rat, the LH-dependent ovarian progesterone rise mediates several actions of the primary surge of LH on the ovary. This experiment was aimed at elucidating the effects of the antiprogestagen RU486 on the LH-dependent decrease in both the serum concentrations and the ovarian content of inhibin. 2. All rats in this experiment were treated with an antagonist of LHRH (1 mg/200 microliters saline at 0800 h in proestrus) to suppress the endogenous release of LH. One group of rats received 32 micrograms LH/250 microliters saline at 1200 h in proestrus. Other group was given 4 mg RU486/200 microliters oil at 0800 h in proestrus. The third group was injected with both RU486 and LH. Rats from the control group were injected with 250 microliters saline and 200 microliters oil. Animals were decapitated at 1700 h in proestrus and trunk blood and ovaries collected to determine the serum concentrations of LH, FSH, progesterone, 17 beta-estradiol and inhibin as well as the ovarian content of inhibin. 3. The ovulatory dose of LH in LHRHa-treated rats decreased both the serum concentrations and the ovarian content of inhibin and increased the serum concentrations of FSH. The administration of RU486 blocked the effect of LH on the serum concentrations of inhibin but not that on the ovarian content of inhibin. 4. Since the antiprogestagen RU486 blocked the effect of LH on the serum concentrations of inhibin, we conclude that ovarian progesterone, besides mediating the effects of the primary LH surge on the ovulatory process and luteinization, participates in the LH-dependent drop in the serum concentrations of inhibin in proestrous afternoon.

摘要

在大鼠中，促黄体生成素（LH）依赖性的卵巢孕酮升高介导了LH初次激增对卵巢的多种作用。本实验旨在阐明抗孕激素RU486对LH依赖性的抑制素血清浓度和卵巢含量降低的影响。2. 本实验中所有大鼠均用促性腺激素释放激素（LHRH）拮抗剂处理（在动情前期08:00时，1毫克/200微升生理盐水）以抑制LH的内源性释放。一组大鼠在动情前期12:00时接受32微克LH/250微升生理盐水。另一组在动情前期08:00时给予4毫克RU486/200微升油剂。第三组同时注射RU486和LH。对照组大鼠注射250微升生理盐水和200微升油剂。在动情前期17:00时将动物断头，收集躯干血液和卵巢，以测定血清中LH、促卵泡生成素（FSH）、孕酮、17β-雌二醇和抑制素的浓度以及卵巢中抑制素的含量。3. 在LHRHa处理的大鼠中，排卵剂量的LH降低了抑制素的血清浓度和卵巢含量，并升高了FSH的血清浓度。给予RU486阻断了LH对抑制素血清浓度的作用，但未阻断对抑制素卵巢含量的作用。4. 由于抗孕激素RU486阻断了LH对抑制素血清浓度的作用，我们得出结论，卵巢孕酮除了介导LH初次激增对排卵过程和黄体化的作用外，还参与了动情前期下午LH依赖性的抑制素血清浓度下降。