血型糖蛋白A跨膜螺旋的丙氨酸插入扫描诱变：一种绘制整合膜蛋白中螺旋-螺旋相互作用的快速方法。
Ala-insertion scanning mutagenesis of the glycophorin A transmembrane helix: a rapid way to map helix-helix interactions in integral membrane proteins.
作者信息
Mingarro I, Whitley P, Lemmon M A, von Heijne G
机构信息
Department of Biochemistry, Stockholm University, Sweden.
出版信息
Protein Sci. 1996 Jul;5(7):1339-41. doi: 10.1002/pro.5560050712.
Abstract
Alanine insertions into the glycophorin A transmembrane helix are found to disrupt helix-helix dimerization in a way that is fully consistent with earlier saturation mutagenesis data, suggesting that Ala-insertion scanning can be used to rapidly map the approximate location of structurally and/or functionally important segments in transmembrane helices.
摘要
研究发现，丙氨酸插入血型糖蛋白A跨膜螺旋中会破坏螺旋-螺旋二聚化，这种方式与早期的饱和诱变数据完全一致，这表明丙氨酸插入扫描可用于快速定位跨膜螺旋中结构和/或功能重要片段的大致位置。
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