Kontturi A K, Kontturi K, Vuoristo M
Laboratory of Physical Chemistry and Electrochemistry, Helsinki University of Technology, Finland.
Acta Chem Scand (Cph). 1996 Feb;50(2):102-6. doi: 10.3891/acta.chem.scand.50-0102.
A method for separating proteins of nearly the same size but having different isoelectric points is presented. The method used is convective electrophoresis, which is based on the simultaneous effects of diffusion, electrical migration and opposing convective flow on the transport of proteins through a porous membrane. The pairs of model proteins used in this study were cytochrome c-lysozyme and human serum albumin-haemoglobin. The obtained results show that with a careful adjustment of pH these proteins can be separated by a continuous manner, and no coupling of protein fluxes could be detected in either case.
本文提出了一种分离大小相近但等电点不同的蛋白质的方法。所采用的方法是对流电泳，它基于扩散、电迁移和相反的对流对蛋白质通过多孔膜传输的同时作用。本研究中使用的模型蛋白质对是细胞色素c-溶菌酶和人血清白蛋白-血红蛋白。所得结果表明，通过仔细调节pH值，这些蛋白质可以连续分离，并且在两种情况下均未检测到蛋白质通量的耦合。
