铜绿假单胞菌基因组DNA序列的分离与鉴定，该序列编码一种属于超家族I的假定DNA解旋酶。
Isolation and characterization of a Pseudomonas aeruginosa genomic DNA sequence, encoding a putative DNA helicase belonging to superfamily I.
作者信息
Avichezer D, Gilboa-Garber N, Katcoff D J
机构信息
Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot, Israel.
出版信息
Microbios. 1995;84(340):187-94.
The isolation and characterization of a 2,122 bp DNA fragment from a Pseudomonas aeruginosa genomic library containing a 1,495 bp open reading frame (ORF) encoding a putative DNA helicase is described. The deduced amino acid (498 residues) sequence derived from this ORF, exhibited a high degree of homology to the rep and uvrD helicases of Escherichia coli (55 and 38% identities, respectively), and to those of Staphylococcus aureus and Haemophilus influenzae (43 and 39% identities, respectively), all of them belonging to superfamily I.
描述了从铜绿假单胞菌基因组文库中分离和鉴定一个2,122 bp的DNA片段，该片段包含一个1,495 bp的开放阅读框（ORF），编码一种假定的DNA解旋酶。从该ORF推导的氨基酸序列（498个残基）与大肠杆菌的rep和uvrD解旋酶具有高度同源性（分别为55%和38%的同一性），与金黄色葡萄球菌和流感嗜血杆菌的解旋酶也具有高度同源性（分别为43%和39%的同一性），它们均属于超家族I。
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