Ghiorse W C, Miller D N, Sandoli R L, Siering P L
Section of Microbiology, Division of Biological Sciences, Cornell University, Ithaca, New York 14853, USA.
Microsc Res Tech. 1996 Jan 1;33(1):73-86. doi: 10.1002/(SICI)1097-0029(199601)33:1<73::AID-JEMT7>3.0.CO;2-1.
Laser scanning microscopy was used 1) to analyze Mn oxide-encrusted biofilms and particles in marine Mn-oxidizing enrichment cultures, 2) to optimize fluorescence in situ hybridization protocols of 16S ribosomal RNA-targeted oligonucleotide signature probes for single bacterial cell identification in particles from a wetland, and 3) to develop a combined immunofluorescence-microautoradiography procedure for analysis of the distribution of 14C-labeled organic compounds and 14C-mineralizing bacteria in groundwater seep sediments. The results demonstrated the wide applicability and benefits of using laser scanning microscopy for analysis of complex microbial assemblages.
1）分析海洋锰氧化富集培养物中被锰氧化物包裹的生物膜和颗粒；2）优化用于识别湿地颗粒中单个细菌细胞的16S核糖体RNA靶向寡核苷酸特征探针的荧光原位杂交方案；3）开发一种免疫荧光-显微放射自显影联合程序，用于分析地下水渗流沉积物中14C标记有机化合物和14C矿化细菌的分布。结果表明，激光扫描显微镜在分析复杂微生物群落方面具有广泛的适用性和优势。
