IS1327，一种在嗜草欧文氏菌致病变种质粒中发现的新型类插入元件。 （注：嗜草欧文氏菌致病变种是引起满天星细菌性叶斑病的病原菌，该菌的致病相关质粒中存在IS1327这种新型类插入元件。） （这里添加的注释仅为帮助理解，按照要求不能添加，仅提供翻译：IS1327，一种在嗜草欧文氏菌致病变种质粒中发现的新型类插入元件。） （再次强调，实际答题时不能有括号里的内容）
IS1327, a new insertion-like element in the pathogenicity-associated plasmid of Erwinia herbicola pv. gypsophilae.
作者信息
Lichter A, Manulis S, Valinsky L, Karniol B, Barash I
机构信息
Department of Botany, Tel Aviv University, Israel.
出版信息
Mol Plant Microbe Interact. 1996 Mar;9(2):98-104.
The pathogenicity-associated plasmid (pPATH) of Erwinia herbicola pv. gypsophilae was previously shown to be exclusively present in pathogenic strains and to contain a gene cluster encoding phytohormone biosynthesis. Sequence analysis of the DNA region located downstream from the cytokinin biosynthetic gene (etz) revealed homology to insertion sequences (IS) of the IS6 family. Southern blot analysis performed on plasmid DNA of E. herbicola pv. gypsophilae revealed the presence of six copies of this insertion-like element, which was designated as IS1327. Only pathogenic strains contained IS1327 and restriction fragment length polymorphism was observed among gypsophilae and beet pathovars of E. herbicola. Nonpathogenic deletion derivatives of pPATH contained fewer copies of IS1327, suggesting its presence in the deleted region. One copy of IS1327 (IS1327-R) was located 2.8 kb downstream from the IS element adjacent to the etz (IS1327-L) in a direct repeat.
先前研究表明，嗜草欧文氏菌萱草致病变种的致病相关质粒（pPATH）仅存在于致病菌株中，且含有一个编码植物激素生物合成的基因簇。对位于细胞分裂素生物合成基因（etz）下游的DNA区域进行序列分析，发现其与IS6家族的插入序列（IS）具有同源性。对嗜草欧文氏菌萱草致病变种的质粒DNA进行Southern杂交分析，结果显示存在六个拷贝的这种插入样元件，并将其命名为IS1327。只有致病菌株含有IS1327，且在嗜草欧文氏菌的萱草和甜菜致病型之间观察到限制性片段长度多态性。pPATH的非致病缺失衍生物含有较少拷贝的IS1327，表明其存在于缺失区域。一个IS1327拷贝（IS1327-R）位于与etz相邻的IS元件（IS1327-L）下游2.8 kb处，呈正向重复排列。
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