大鼠肾上腺铁氧化还原蛋白全长cDNA的克隆与序列分析

Cloning and sequence analysis of a full-length cDNA of rat adrenodoxin.

作者信息

Sagara Y, Watanabe Y, Kawamura K, Yubisui T

机构信息

Department of Medical Biology, Kochi Medical School, Japan.

出版信息

Biol Pharm Bull. 1996 Jan;19(1):39-41. doi: 10.1248/bpb.19.39.

DOI:10.1248/bpb.19.39

PMID:8820908

Abstract

A cDNA clone which covers the entire coding region for the precursor of adrenodoxin was isolated from a rat adrenal cDNA library. This precursor consists of amino-terminal 64 residues of extrapeptide for transport into mitochondria and the following 124 residues of mature peptide region. The amino acid sequence of rat mature adrenodoxin showed 85-98% homology with mouse, human, chicken, porcine, bovine and sheep counterparts, whereas that of the extrapeptide showed significantly lower values.

摘要

从大鼠肾上腺cDNA文库中分离出一个覆盖肾上腺皮质铁氧还蛋白前体整个编码区的cDNA克隆。该前体由用于转运到线粒体的64个氨基酸残基的氨基末端额外肽段和随后的124个氨基酸残基的成熟肽区域组成。大鼠成熟肾上腺皮质铁氧还蛋白的氨基酸序列与小鼠、人类、鸡、猪、牛和羊的对应序列具有85%-98%的同源性，而额外肽段的同源性则明显较低。