Sohma O, Mizuguchi M, Takashima S, Yamada M, Ikeda K, Ohta S
Department of Mental Retardation and Birth Defect Research, National Institute of Neuroscience, Tokyo, Japan.
J Neurosci Res. 1996 Jan 15;43(2):175-82. doi: 10.1002/(SICI)1097-4547(19960115)43:2<175::AID-JNR5>3.0.CO;2-D.
The gene bcl-x, which is related to a bcl-2, regulates programmed cell death. bcl-x may function in the development of the nervous system. We raised a polyclonal antibody against human Bcl-x protein, and investigated its distribution in the developing human cerebellum. Western blotting revealed that Bcl-x expression in the cerebellum is higher in the fetal, than in the postnatal period. Immunohistochemical studies of fetal brains localized intense Bcl-x immunoreactivity in the granule cell processes at 13-22 gestational weeks and in the Purkinje cell bodies at 24-38 weeks. The immunoreactivity decreased after birth, but was retained in the Purkinje cells at a low level until adulthood. These results suggested that Bcl-x expression in the cerebellum is developmentally regulated and involved specifically in the development of neuronal subpopulations.
与bcl-2相关的基因bcl-x调节程序性细胞死亡。bcl-x可能在神经系统发育中发挥作用。我们制备了一种针对人Bcl-x蛋白的多克隆抗体，并研究了其在发育中的人类小脑中的分布。蛋白质印迹分析显示，小脑中小脑Bcl-x的表达在胎儿期高于出生后。对胎儿大脑的免疫组织化学研究表明，在妊娠13-22周时，颗粒细胞突起中有强烈的Bcl-x免疫反应性，在24-38周时，浦肯野细胞体中有强烈的Bcl-x免疫反应性。出生后免疫反应性降低，但在浦肯野细胞中一直保持低水平直至成年。这些结果表明，小脑中Bcl-x的表达受发育调控，并特别参与神经元亚群的发育。
