Jander S, Pohl J, Gillen C, Stoll G
Department of Neurology, Heinrich-Heine-University, Düsseldorf, Germany.
J Neurosci Res. 1996 Jan 15;43(2):254-9. doi: 10.1002/(SICI)1097-4547(19960115)43:2<254::AID-JNR13>3.0.CO;2-6.
Interleukin-10 (IL-10) is a potent immunosuppressant cytokine which downregulates MHC class II antigen expression and inflammatory cytokine production. In this study we localized mRNA for IL-10 in the rat peripheral nervous system (PNS) by nonradioactive in situ hybridization using a digoxygenin-labeled riboprobe specific for rat IL-10. IL-10 mRNA was expressed by some Schwann cells (SCs) in the normal sciatic nerve. During Wallerian degeneration, SCs strongly expressed IL-10 mRNA between days 2 and 4 after transection. By day 14 only occasional cells were positive for IL-10 mRNA. The vast majority of ED1-positive macrophages were IL-10 negative after axotomy. Contrastingly, infiltrating macrophages expressed IL-10 mRNA conincident with beginning clinical recovery in experimental autoimmune neuritis (EAN), the rat model of human Guillain-Barré syndrome. Our data suggest that SCs provide a constitutive immunosuppressant system in the PNS. In EAN additional macrophage-derived IL-10 may be important for the resolution of the T cell-mediated immune response.
白细胞介素-10（IL-10）是一种强效免疫抑制细胞因子，可下调II类主要组织相容性复合体（MHC）抗原表达及炎性细胞因子的产生。在本研究中，我们使用地高辛标记的、对大鼠IL-10特异的核糖探针，通过非放射性原位杂交技术，对大鼠外周神经系统（PNS）中的IL-10信使核糖核酸（mRNA）进行定位。正常坐骨神经中的一些雪旺细胞（SCs）表达IL-10 mRNA。在华勒氏变性过程中，横断后第2至4天，雪旺细胞强烈表达IL-10 mRNA。到第14天时，只有偶尔的细胞IL-10 mRNA呈阳性。绝大多数ED1阳性巨噬细胞在轴突切断后IL-10呈阴性。相反，在实验性自身免疫性神经炎（EAN，人类格林-巴利综合征的大鼠模型）中，浸润的巨噬细胞在临床开始恢复时表达IL-10 mRNA。我们的数据表明，雪旺细胞在PNS中提供了一个组成性免疫抑制系统。在EAN中，额外的巨噬细胞源性IL-10对于T细胞介导的免疫反应的消退可能很重要。
