Bataille N, Vallancien G, Chopin D
Department of Urology, Centre d'Etude et de Recherche appliquée, CMC de la Porte de Choisy, Paris, France.
Eur Urol. 1996;29(1):72-7. doi: 10.1159/000473722.
We evaluated the effect of focused extracorporeal pyrotherapy on local tumor growth and metastatic potential of the Dunning R-3327 tumor inoculated into male Copenhagen Fisher rats, using the highly metastatic MAT Ly Lu cell line in 64 rats and the slightly metastatic G cell line in 60 rats. Five million cells were inoculated subcutaneously into a lower limb and the tumor volume was determined every 48 h. The local effect, the presence of lung and/or lymph node metastases and the spontaneous survival were evaluated in the treated groups (n = 30 for MAT Ly Lu and n = 29 for G) and in the sham control groups (n = 19 for MAT Ly Lu and n = 13 for G) by autopsy of the animals at successive times after treatment. A local effect of treatment was obtained for the two cell lines with complete local cure in 53% of cases. The animals developing metastases were not more numerous in the treated groups than in the control groups. Long-term survivals were obtained in the animals inoculated with the two cell lines. These results suggest that pyrotherapy exerts a local effect on the Dunning R-3327 tumor model without increasing its metastatic potential and allows long-term survivals. Controlled therapeutic trials in human cancers are envisaged.
我们评估了聚焦体外热疗对接种于雄性哥本哈根费希尔大鼠体内的邓宁R - 3327肿瘤局部生长和转移潜能的影响，其中64只大鼠使用高转移性MAT Ly Lu细胞系，60只大鼠使用低转移性G细胞系。将500万个细胞皮下接种到下肢，每48小时测定一次肿瘤体积。通过在治疗后的连续时间对动物进行尸检，评估治疗组（MAT Ly Lu组n = 30，G组n = 29）和假手术对照组（MAT Ly Lu组n = 19，G组n = 13）的局部效应、肺和/或淋巴结转移情况以及自然生存期。两种细胞系均获得了局部治疗效果，53%的病例实现了局部完全治愈。治疗组中发生转移的动物数量并不比对照组多。接种两种细胞系的动物均获得了长期生存。这些结果表明，热疗对邓宁R - 3327肿瘤模型具有局部作用，且不会增加其转移潜能，并能实现长期生存。设想在人类癌症中进行对照治疗试验。
