Ohara S, Beaudet L N, Schmidt R E
Department of Pathology, Neuropathology, Washington University School of Medicine, Saint Louis, MO 63110, USA.
Brain Res. 1995 Dec 24;705(1-2):325-31. doi: 10.1016/0006-8993(95)01164-1.
Sciatic nerve axotomy induces the transganglionic expression of the growth associated protein GAP-43 and neuropeptide Y (NPY) in lumbar DRG projections to the gracile nucleus. Four weeks after axotomy young animals had developed delicate GAP-43 and NPY-immunoreactive axonal sprouts in the gracile nuclei; however, an identical insult to aged (14-26 months) animals resulted in the labeling of swollen dystrophic elements and fewer delicate axonal sprouts. Unilateral sciatic transection in young rats with subsequent frustration of regeneration for 8 months resulted in ipsilateral gracile neuroaxonal dystrophy as assessed by ultrastructural, immunohistologic and quantitative morphometric techniques.
坐骨神经切断术可诱导生长相关蛋白GAP - 43和神经肽Y（NPY）在腰段背根神经节向薄束核投射中的跨节表达。切断术后四周，幼龄动物在薄束核中已形成纤细的GAP - 43和NPY免疫反应性轴突发芽；然而，对老龄（14 - 26个月）动物进行相同损伤后，结果是肿胀的营养不良性成分被标记，且纤细的轴突发芽较少。通过超微结构、免疫组织化学和定量形态计量学技术评估，幼龄大鼠单侧坐骨神经横断并随后阻碍再生8个月，导致同侧薄束核神经轴突营养不良。
