[秋水仙碱对地中海伞藻形态发生及超微结构的影响]

[Effect of colchicine on the morphogenesis and ultrastruct of Acetabularia mediterranea].

作者信息

Betina M I, Salamakha O V, Tikhomirova L A, Ianushevich I V

出版信息

Ontogenez. 1977;8(1):62-7.

Abstract

The effect of colchicine on morphogenesis of the regenerating nuclear fragments of Acetabularia mediterranea has been studied. Colchicine was shown to inhibit the formation of caps. The algae are most sensitive to colchicine at the early stages of regeneration. The breakdown of microtubules in the perinuclear cytoplasm of regenerating algae under the effect of colchicine was found under the electron microscopy. The breakdown of microtubules in this zone appears not to disturb the transport of morphogenetical substances in the apical cell part. Colchicine inhibits the morphogenesis, apparently, at the expense of protein inactivation in the zone of growth.

摘要

已经研究了秋水仙碱对地中海伞藻再生核片段形态发生的影响。结果表明，秋水仙碱会抑制帽状体的形成。藻类在再生早期对秋水仙碱最为敏感。在电子显微镜下发现，秋水仙碱作用下再生藻类核周细胞质中的微管会解体。该区域微管的解体似乎并未干扰顶端细胞部分形态发生物质的运输。显然，秋水仙碱抑制形态发生是通过使生长区域的蛋白质失活来实现的。