Fukamauchi F, Mataga N, Wang Y J, Chuang D M
Department of Molecular Medical Science, Tokyo Medical and Dental University, Japan.
Neurosci Lett. 1996 Jul 5;212(1):49-52. doi: 10.1016/0304-3940(96)12779-8.
NCB-20 cells expressed m1- and m4-muscarinic acetylcholine receptor (mAChR) mRNAs, while NG108-15 cells expressed only m4-mAChR mRNA. Butyrate induced a time-dependent increase in the level of m1-mAChR mRNA with no change in the m4-mAChR mRNA level in NCB-20 cells. Similarly, butyrate did not affect the m4-mAChR mRNA level in NG108-15 cells. In contrast, dibutyryl cAMP caused a significant time-dependent decrease in the level of m4-mAChR mRNA in NCB-20 and NG108-15 cells as well as m1-mAChR mRNA in NCB-20 cells. Our results suggest that these two differentiating agents are important physiological regulators of the transcription and/or stability of the mRNA of certain mAChR subtypes expressed in these two neurohybrid cell lines.
NCB - 20细胞表达m1和m4毒蕈碱型乙酰胆碱受体（mAChR）的mRNA，而NG108 - 15细胞仅表达m4 - mAChR的mRNA。丁酸盐使NCB - 20细胞中m1 - mAChR mRNA水平呈时间依赖性增加，而m4 - mAChR mRNA水平无变化。同样，丁酸盐对NG108 - 15细胞中的m4 - mAChR mRNA水平也无影响。相反，二丁酰环磷腺苷（dibutyryl cAMP）使NCB - 20和NG108 - 15细胞中m4 - mAChR mRNA水平以及NCB - 20细胞中m1 - mAChR mRNA水平出现显著的时间依赖性降低。我们的结果表明，这两种分化剂是这两种神经杂交细胞系中表达的某些mAChR亚型mRNA转录和/或稳定性的重要生理调节因子。
