Ku K, Nosaka S, Hashimoto M, Kin S, Saitoh Y, Alam M S, Masumura S, Nakayama K
First Department of Surgery, Shimane Medical University, Izumo, Japan.
Transplantation. 1996 Sep 27;62(6):735-42. doi: 10.1097/00007890-199609270-00006.
We examined the effects of supplementation with eiosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), major components of omega-3 polyunsaturated (correction of polyunsatulated) fatty acids (PUFAs), on basal cardiac function and recovery of cardiac function of "donor hearts" from adults (30 week) rats following cold preservation and reperfusion (P/R). In groups 1, 2, 3, and 4, respectively, 30-week-old rats were fed a soybean oil diet, a high-cholesterol oil (HC) diet, an HC diet with EPA, or an HC diet with DHA for 5 weeks. After collecting blood to analyze plasma levels of fatty acids among each group, the heart was excised and perfused on a Langendorff apparatus. Following evaluation of each rat's cardiac function, each heart was stored in HTK solution for 8 hr at 4 degrees C. The heart was then reperfused and the coronary perfusate was collected to evaluate enzyme that had leaked. After cardiac functional recovery was estimated, myocardial fatty acids were measured. EPA supplementation significantly increases the plasma and cardiac levels of EPA as well as the ratio of EPA to arachidonic acid (AA). EPA supplementation also led to improved recovery of cardiac function following P/P, compared with that of rats who received soybean oil, high-cholesterol oil, and DHA. DHA supplementation significantly increased the plasma and cardiac levels of DHA as well as the ratio of DHA to AA--however, the cardiac functional recovery was almost identical to that of the rats who received high-cholesterol oil and was higher only than that of the rats who received soybean oil. There were no significant differences in enzyme that had leaked and myocardial water content among each group. These results suggest that alterations in the myocardial phospholipid composition by EPA supplementation may be profoundly responsible for attenuating myocardial I/R injuries. In contrast, DHA supplementation may not exert a cardioprotective effect following cold P/R. DHA supplementation alone may not increase the myocardial level of EPA enough to cause a protective effect against P/R injury. EPA supplementation to hyperlipidemic patients may be clinically warranted for increasing the potential number of donors.
我们研究了补充ω-3多不饱和脂肪酸（PUFAs）的主要成分二十碳五烯酸（EPA）和二十二碳六烯酸（DHA），对成年（30周龄）大鼠“供体心脏”在冷保存和再灌注（P/R）后基础心脏功能及心脏功能恢复的影响。在第1、2、3和4组中，30周龄大鼠分别喂食大豆油饮食、高胆固醇油（HC）饮食、含EPA的HC饮食或含DHA的HC饮食5周。在采集血液分析每组的血浆脂肪酸水平后，取出心脏并在Langendorff装置上进行灌注。在评估每只大鼠的心脏功能后，将每个心脏在4℃下于HTK溶液中保存8小时。然后对心脏进行再灌注，并收集冠状动脉灌注液以评估渗漏的酶。在估计心脏功能恢复后，测量心肌脂肪酸。补充EPA显著增加了血浆和心脏中的EPA水平以及EPA与花生四烯酸（AA）的比率。与接受大豆油、高胆固醇油和DHA的大鼠相比，补充EPA还导致P/P后心脏功能恢复得到改善。补充DHA显著增加了血浆和心脏中的DHA水平以及DHA与AA的比率——然而，心脏功能恢复与接受高胆固醇油的大鼠几乎相同，仅高于接受大豆油的大鼠。每组之间渗漏的酶和心肌含水量没有显著差异。这些结果表明，补充EPA引起的心肌磷脂组成变化可能是减轻心肌I/R损伤的主要原因。相比之下，补充DHA在冷P/R后可能不会发挥心脏保护作用。单独补充DHA可能不会使心肌中的EPA水平增加到足以对P/R损伤产生保护作用的程度。对高脂血症患者补充EPA在临床上可能有助于增加潜在供体数量。
