Lang T, Krams S M, Martinez O M
Transplantation Immunobiology Laboratory, California Pacific Medical Center, San Francisco 94115, USA.
Transplantation. 1996 Sep 27;62(6):776-80. doi: 10.1097/00007890-199609270-00014.
The delineation of T helper cell subsets into T helper type 1 (Th1) and T helper type 2 (Th2) populations based on the production of specific cytokines has been useful in understanding the regulation and progression of immune-based pathologies. In order to test the relevance of this concept to human solid organ transplantation, in situ and system Th1 and Th2 cytokine profiles were characterized in liver allograft recipients. Bile and serum samples obtained posttransplant were analyzed for the cytokines IL-2, IFN-gamma, IL-4, and IL-10. Significant elevations of IL-4 and IL-10 were measured at the site of graft rejection. In contrast, IL-2, IFN-gamma, and IL-4 were markedly elevated in the circulation during rejection. Analyses of sequential bile samples revealed that Th1 and Th2 cytokines showed similar kinetics of production in response to alloantigen. Taken together, these results indicate that acute rejection of human allografts can proceed [correction of procede] in the presence of minimal levels of the Th1 cytokines IL-2 and IFN-gamma and high levels of IL-4 and IL-10.
基于特定细胞因子的产生将辅助性T细胞亚群划分为1型辅助性T细胞（Th1）和2型辅助性T细胞（Th2）群体，这对于理解基于免疫的病理状况的调节和进展很有帮助。为了检验这一概念与人类实体器官移植的相关性，对肝移植受者的原位和全身Th1和Th2细胞因子谱进行了表征。分析移植后获得的胆汁和血清样本中的细胞因子白细胞介素-2（IL-2）、干扰素-γ（IFN-γ）、白细胞介素-4（IL-4）和白细胞介素-10（IL-10）。在移植排斥部位检测到IL-4和IL-10显著升高。相反，在排斥反应期间，循环中的IL-2、IFN-γ和IL-4显著升高。对连续胆汁样本的分析表明，Th1和Th2细胞因子在对同种异体抗原的反应中表现出相似的产生动力学。综上所述，这些结果表明，在Th1细胞因子IL-2和IFN-γ水平极低而IL-4和IL-10水平较高的情况下，人类同种异体移植的急性排斥反应仍可发生。
