Apone L M, Virbasius C M, Reese J C, Green M R
Howard Hughes Medical Institute, University of Massachusetts Medical Center, Worcester, Massachusetts 01605, USA.
Genes Dev. 1996 Sep 15;10(18):2368-80. doi: 10.1101/gad.10.18.2368.
The RNA polymerase II general transcription factor TFIID is a multisubunit complex comprising TATA-box binding protein and associated factors (TAFIIs). In vitro experiments have suggested that TAFIIs are essential coactivators required for RNA polymerase II-directed transcription activation. Here, for the first time, we analyze systematically the in vivo function of a specific TAFII, yeast TAFII90 (yTAFII90). We show that functional inactivation of yTAFII90 by temperature-sensitive mutations or depletion leads to arrest at the G2/M phase of the cell cycle. Unexpectedly, in the absence of functional yTAFII90, a variety of endogenous yeast genes were all transcribed normally, including those driven by well-characterized activators. Taken together, our results indicate that yTAFII90 is not required for transcription activation in general, and reveal linkages between TAF function and cell-cycle progression.
RNA聚合酶II通用转录因子TFIID是一种多亚基复合物，由TATA框结合蛋白和相关因子（TAFIIs）组成。体外实验表明，TAFIIs是RNA聚合酶II介导的转录激活所必需的共激活因子。在此，我们首次系统分析了特定TAFII——酵母TAFII90（yTAFII90）的体内功能。我们发现，温度敏感突变或缺失导致yTAFII90功能失活会使细胞周期停滞在G2/M期。出乎意料的是，在缺乏功能性yTAFII90的情况下，多种内源性酵母基因均能正常转录，包括那些由特征明确的激活因子驱动的基因。综上所述，我们的结果表明，一般转录激活并不需要yTAFII90，并揭示了TAF功能与细胞周期进程之间的联系。
