Stivala L A, Scovassi A I, Bianchi L, Prosperi E
Istituto di Patologia Generale C Golgi, Università di Pavia, Italy.
Biochimie. 1995;77(11):888-92. doi: 10.1016/0300-9084(95)90008-x.
We have investigated the cell cycle-dependent nuclear binding of cyclin A and of the proliferating cell nuclear antigen (PCNA) in asynchronously growing human fibroblasts. To this purpose, we have applied flow cytometry immunofluorescence, a powerful technique for elucidating the cell cycle phase during which the nuclear binding occurs. We have observed that, in striking contrast with the distribution of nuclear-bound PCNA which is restricted to S phase, the immunofluorescence signal of the nuclear-bound form of cyclin A is high in the G1 and G2 phases of the cell cycle. These results suggest the involvement of nuclear-bound cyclin A in the G1/S and G2/M phase transitions.
我们研究了细胞周期蛋白A和增殖细胞核抗原（PCNA）在异步生长的人成纤维细胞中的细胞周期依赖性核结合。为此，我们应用了流式细胞术免疫荧光技术，这是一种用于阐明核结合发生的细胞周期阶段的强大技术。我们观察到，与仅限于S期的核结合PCNA的分布形成鲜明对比的是，细胞周期蛋白A的核结合形式的免疫荧光信号在细胞周期的G1和G2期很高。这些结果表明核结合的细胞周期蛋白A参与了G1/S和G2/M期转换。
