Takahara K, Osborne L, Elliott R W, Tsui L C, Scherer S W, Greenspan D S
Department of Pathology and Laboratory Medicine, University of Wisconsin Medical School, Madison 53706, USA.
Genomics. 1996 Jan 15;31(2):253-6. doi: 10.1006/geno.1996.0043.
The human gene (PCOLCE) for the C-proteinase enhancer, a glycoprotein that enhances the enzymatic activity that cleaves the type I procollagen C-propeptide, was previously mapped to the same cytogenetic region (7q21.3-q22) as COL1A2, the type I collagen pro-alpha 2 chain gene. The apparent proximity of these two genes, whose products interact, might have reflected some mechanism for coregulation of expression. We have employed somatic cell hybrids, cosmid contigs, and interspecific backcross mice to investigate further the apparent proximity of the two genes. Our data show that PCOLCE and COL1A2 are separated by at least 6 Mb on human chromosome 7q and that Pcolce and Col1a2 are located on two separate chromosomes in mouse. These data also indicate that PCOLCE is located within a 10- to 15-cM region of conserved synteny between human chromosome 7 and mouse chromosome 5.
C蛋白酶增强子的人类基因（PCOLCE）是一种糖蛋白，可增强切割I型前胶原C前肽的酶活性，该基因先前被定位到与I型胶原原α2链基因COL1A2相同的细胞遗传学区域（7q21.3 - q22）。这两个基因的产物相互作用，它们明显相邻，这可能反映了某种表达共调控机制。我们利用体细胞杂种、黏粒重叠群和种间回交小鼠进一步研究这两个基因的明显相邻关系。我们的数据表明，在人类7号染色体上，PCOLCE和COL1A2至少相隔6 Mb，并且在小鼠中，Pcolce和Col1a2位于两条不同的染色体上。这些数据还表明，PCOLCE位于人类7号染色体和小鼠5号染色体之间保守同线性的10至15厘摩区域内。
