Soon L L, Breed W G
Department of Anatomy and Histology, University of Adelaide, Australia.
Mol Reprod Dev. 1996 Feb;43(2):217-27. doi: 10.1002/(SICI)1098-2795(199602)43:2<217::AID-MRD11>3.0.CO;2-0.
In the dasyurid marsupial, Sminthopsis crassicaudata, the mature spermatozoon has an inner homogeneous (C1) and a peripheral indented (C2) region. Using DNase-gold conjugates, and biotinylated genomic DNA probes, DNA was found to occur in both C1 and C2 regions. The morphogenesis of the spermatozoon nucleus was investigated using ultrastructural and cytochemical studies. Spermiogenesis was divided into 15 steps. By step 10, condensation of the C1 region was complete, and at the caudal extremity of the spermatid nucleus, the nuclear envelope enclosed an electron-lucent space. This space and the surrounding nuclear envelope became very enlarged at step 11. At this stage, a plate of approximately 70 nm in thickness was present along the caudal segment of the C1 region; this "nuclear mantle" did not bind DNase-gold conjugates but stained for lysine-rich proteins using alcoholic phosphotungstic acid. Chromatin condensation resumed at step 12 with the appearance of spherical chromatin structures peripheral to the C1 chromatin. These structures then partially coalesced and the indentations of the C2 region were observed. The expanded nuclear envelope at the caudal extremity persisted in caput epididymal spermatozoa. Spherical inclusions within it did not bind to DNase-gold conjugates but stained for lysine-rich proteins. As the sperm traveled down the epididymis, these inclusions amassed near the nuclear pores and were then removed from the nucleus. In addition, the nuclear mantle was found to have disappeared by the time the spermatozoa reached the corpus epididymidis.
在袋鼬科有袋动物肥尾袋鼩（Sminthopsis crassicaudata）中，成熟精子有一个内部均匀的（C1）区域和一个周边呈锯齿状的（C2）区域。使用脱氧核糖核酸酶 - 金复合物和生物素化的基因组DNA探针，发现DNA存在于C1和C2区域。利用超微结构和细胞化学研究对精子细胞核的形态发生进行了研究。精子发生分为15个阶段。到第10阶段时，C1区域的浓缩完成，在精子细胞核的尾端，核膜包围着一个电子透明空间。在第11阶段，这个空间和周围的核膜变得非常膨大。在此阶段，沿着C1区域的尾段有一块厚度约为70纳米的板；这个“核套”不与脱氧核糖核酸酶 - 金复合物结合，但用乙醇磷钨酸对富含赖氨酸的蛋白质进行染色。在第12阶段，随着C1染色质周边出现球形染色质结构，染色质浓缩恢复。这些结构随后部分合并，并观察到C2区域的凹陷。附睾头精子的尾端膨大的核膜持续存在。其中的球形内含物不与脱氧核糖核酸酶 - 金复合物结合，但对富含赖氨酸的蛋白质进行染色。随着精子沿附睾下行，这些内含物在核孔附近聚集，然后从细胞核中被清除。此外，发现当精子到达附睾体时核套已经消失。
