Layman L R, Dory A C, Koehnlein K M, Fried B, Sherma J
Department of Chemistry, Lafayette College, Easton, PA 18042, USA.
Parasitol Res. 1996;82(1):19-21. doi: 10.1007/s004360050061.
Flame and graphite-furnace atomic absorption spectrometry and inductively coupled plasma atomic emission spectrometry were used to study the metallic ions in the digestive gland-gonad complex (DGG) of Helisoma trivolvis snails infected with the daughter rediae of Echinostoma trivolvis and in uninfected DGG. Seven metals were found to be present in infected and uninfected DGG at concentrations above the detection limits of the analytical methods. Of these, sodium was present in significantly higher amounts (Student's t-test, confidence level of 95%) in the infected versus uninfected DGG; magnesium and manganese occurred in significantly lower amounts in the infected DGG. Our results were compared with those from a Bulgarian study in which neutron activation analysis was used to determine elements in Lymnaea stagnalis snails infected with the intramolluscan stages of the 37-collar-spined echinostome E. revolutum. The Bulgarian study also reported a significant elevation of sodium but reduction of zinc in the hepatopancreas (i.e., digestive gland) of infected snails. Other differences between the two studies are discussed.
采用火焰和石墨炉原子吸收光谱法以及电感耦合等离子体原子发射光谱法，研究感染三带棘口吸虫子雷蚴的三角帆蚌消化腺 - 性腺复合体（DGG）和未感染的DGG中的金属离子。结果发现，感染和未感染的DGG中均存在七种金属，其浓度高于分析方法的检测限。其中，感染的DGG中钠的含量明显更高（学生t检验，置信水平为95%）；感染的DGG中镁和锰的含量明显更低。我们将研究结果与保加利亚一项研究的结果进行了比较，该研究采用中子活化分析来测定感染37环棘口吸虫卷棘口吸虫软体动物期的静水椎实螺肝胰腺（即消化腺）中的元素。保加利亚的研究还报告称，感染蜗牛的肝胰腺中钠含量显著升高，但锌含量降低。讨论了两项研究之间的其他差异。
