Santoro T, Maguire J, McBride O W, Avraham K B, Copeland N G, Jenkins N A, Kelly K
Laboratory of Pathology, National Cancer Institute, Bethesda, Maryland 20892, USA.
Genomics. 1995 Dec 10;30(3):558-64. doi: 10.1006/geno.1995.1277.
The mitogen-induced gene, GEM, encodes a GTP-binding protein that belongs to a new family within the Ras superfamily. The regulated expression pattern of Gem suggests a role for this protein in cellular responses to growth stimulation. To facilitate the assessment of the possible role of GEM in heritable and spontaneous disease processes, the genomic organization of human GEM and the chromosomal localization of human and murine GEM have been determined. GEM has been localized to the long arm of human chromosome 8 (8q13-q21) between the D8S85 and CA2 loci by genetic linkage analysis using an MspI restriction fragment length polymorphism within GEM. No consistent somatic chromosomal alterations or heritable diseases are associated with this region. Mouse Gem maps to the proximal region of chromosome 4 between Mos and Cga. To gain insight into the transcriptional regulation of GEM, we have established the transcriptional initiation site of GEM in human T cells and defined a 5' upstream region sufficient for mitogen-responsive, inducible transcription.
有丝分裂原诱导基因GEM编码一种GTP结合蛋白，该蛋白属于Ras超家族中的一个新家族。Gem的表达模式受调控，这表明该蛋白在细胞对生长刺激的反应中发挥作用。为了便于评估GEM在遗传性和自发性疾病过程中可能发挥的作用，已确定了人类GEM的基因组结构以及人类和小鼠GEM的染色体定位。通过使用GEM内的MspI限制性片段长度多态性进行遗传连锁分析，GEM已被定位到人类8号染色体长臂（8q13-q21）上D8S85和CA2基因座之间。该区域未发现一致的体细胞染色体改变或遗传性疾病。小鼠Gem定位于4号染色体近端Mos和Cga之间的区域。为了深入了解GEM的转录调控，我们确定了人类T细胞中GEM的转录起始位点，并定义了一个足以进行有丝分裂原反应性、诱导性转录的5'上游区域。
