重组辛德毕斯病毒在蚊子中的复制与表达。

Replication and expression of a recombinant Sindbis virus in mosquitoes.

作者信息

Rayms-Keller A, Powers A M, Higgs S, Olson K E, Kamrud K I, Carlson J O, Beaty B J

机构信息

Department of Microbiology, Colorado State University, Fort Collins, USA.

出版信息

Insect Mol Biol. 1995 Nov;4(4):245-51. doi: 10.1111/j.1365-2583.1995.tb00030.x.

DOI:10.1111/j.1365-2583.1995.tb00030.x

PMID:8825762

Abstract

A recombinant Sindbis virus, TE/3'2J/ANTI-S, containing LaCrosse virus small segment cDNA in antisense orientation, was inoculated into Aedes triseriatus mosquitoes. Virus replication and LAC-ANTI-S RNA expression were analysed temporally and spatially. TE/3'2J/ANTI-S virus titre peaked at 5.0 log10 TCID50 in heads 6-9 days post infection (p.i.) and decreased to 3.4 log10 TCID50 by 37 days p.i. Salivary glands contained 4.4 log10 TCID50 of virus 6 days p.i.; titres were lower in other organs. LAC-ANTI-S RNA levels paralleled virus titre. SIN E1 antigen was detected in many mosquito organ systems, but in specific cells and tissues of some organs. TE/3'2J/ANTI-S virus exhibited different cellular tropisms in salivary glands of Aedes and Culex mosquitoes.

摘要

一种重组辛德毕斯病毒TE/3'2J/ANTI-S，其含有反义方向的拉科罗斯病毒小片段cDNA，被接种到三带喙库蚊中。对病毒复制和LAC-ANTI-S RNA表达进行了时空分析。TE/3'2J/ANTI-S病毒滴度在感染后（p.i.）6-9天在头部达到峰值5.0 log10 TCID50，并在感染后37天降至3.4 log10 TCID50。感染后6天，唾液腺中含有4.4 log10 TCID50的病毒；其他器官中的滴度较低。LAC-ANTI-S RNA水平与病毒滴度平行。在许多蚊虫器官系统中检测到辛德毕斯病毒E1抗原，但在某些器官的特定细胞和组织中也有检测到。TE/3'2J/ANTI-S病毒在伊蚊和库蚊的唾液腺中表现出不同的细胞嗜性。