Ripoll C, Martin F, Manuel Rovira J, Pintor J, Miras-Portugal M T, Soria B
Department of Physiology, University of Alicante, School of Medicine, Spain.
Diabetes. 1996 Oct;45(10):1431-4. doi: 10.2337/diab.45.10.1431.
Diadenosine polyphosphates are a group of low-weight compounds that increase after exposure to a wide variety of oxidants and have been suggested to act as "alarmones," alerting the cell to the onset of metabolic stress. We demonstrate here that glucose at concentrations that induce insulin release produce a 30- to 70-fold increase in the concentration of diadenosine triphosphate (Ap3A) and tetraphosphate (Ap4A) in beta-cells. Furthermore, Ap3A and Ap4A, at the concentrations found in glucose-stimulated cells, are effective inhibitors of the ATP-regulated K+ channels when applied to the intracellular side of excised membrane patches from cultured beta-cells. We suggest that Ap3A and Ap4A act as second messengers mediating a glucose-induced blockade of the pancreatic beta-cell ATP-regulated potassium channel.
二腺苷多磷酸是一组低分子量化合物，在暴露于多种氧化剂后会增加，并且被认为可作为“警报素”，提醒细胞代谢应激的开始。我们在此证明，诱导胰岛素释放的葡萄糖浓度会使β细胞中二腺苷三磷酸（Ap3A）和四磷酸（Ap4A）的浓度增加30至70倍。此外，当应用于从培养的β细胞中切除的膜片的细胞内侧时，葡萄糖刺激细胞中发现的浓度的Ap3A和Ap4A是ATP调节的钾通道的有效抑制剂。我们认为Ap3A和Ap4A作为第二信使介导葡萄糖诱导的胰腺β细胞ATP调节钾通道的阻断。
