Ayyagari R, Nestorowicz A, Li Y, Chandrasekharappa S, Chinault C, van Tuinen P, Smith R J, Hejtmancik J F, Permutt M A
National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
Genome Res. 1996 Jun;6(6):504-14. doi: 10.1101/gr.6.6.504.
The Usher syndrome type 1C (USH1C) and familial hyperinsulinism (HI) loci have been assigned to chromosome 11p14-15.1, within the interval D11S419-D11S1310. We have constructed a yeast artificial chromosome (YAC) contig, extending from D11S926 to D11S899, which encompasses the critical regions for both USH1C and HI and spans an estimated genetic distance of approximately 4 cM. A minimal set of six YAC clones constitute the contig, with another 22 YACs confirming the order of sequence-tagged sites (STSs) and position of YACs on the contig. A total of 40 STSs, including 10 new STSs generated from YAC insert-end sequences and inter-Alu PCR products, were used to order the clones within the contig. This physical map provides a resource for identification of gene transcripts associated with USH1C, HI, and other genetic disorders that map to the D11S926-D11S899 interval.
1型Usher综合征（USH1C）和家族性高胰岛素血症（HI）基因座已被定位到11号染色体的p14 - 15.1区域，位于D11S419 - D11S1310区间内。我们构建了一个酵母人工染色体（YAC）重叠群，从D11S926延伸至D11S899，它包含USH1C和HI的关键区域，估计跨越约4厘摩的遗传距离。由六个YAC克隆组成的最小重叠群，另有22个YAC用于确认序列标签位点（STS）的顺序以及YAC在重叠群上的位置。总共40个STS，包括从YAC插入末端序列和Alu间PCR产物产生的10个新STS，用于排列重叠群内的克隆。这个物理图谱为鉴定与USH1C、HI以及其他定位到D11S926 - D11S899区间的遗传疾病相关的基因转录本提供了资源。
