The effects of hypothyroidism on single fibers of the rat soleus muscle.

Skinned single fibers were used to test the hypothesis that skinned fibers from hypothyroid soleus muscle would have a higher sensitivity to calcium compared with control fibers, as indicated by a leftward shift of the pCa-force curve. Control rats (n = 14) received sham injections, while the hypothyroid group (n = 16) received thyroidectomy and a 6-week injection period of the antithyroid drug 6-n-propyl-2-thiouracil. Hypothyroidism caused the type I fiber number to increase significantly (11%) in the soleus. Hypothyroid fibers produced 16% less absolute tension than control fibers. However, cross-sectional areas of control fibers were significantly greater (25%) than those of hypothyroid fibers, so that when force was normalized to cross-sectional area, no differences between groups existed. Calcium requirement for half-maximal force production (pCa50) did not differ, but the slope of the pCa-force curve was different between groups. These data suggest that hypothyroidism did not alter the intrinsic force-generating capacity of the soleus muscle fibers. Thus, alterations in hypothyroid soleus contractile function seen in vitro may be explained by alterations in excitation-contraction coupling and (or) shifts in muscle fiber types.