Slunga-Tallberg A, el-Rifai W, Keinänen M, Ylinen K, Kurki T, Klinger K, Ylikorkala O, Larramendy M L, Knuutila S
Department of Medical Genetics, University of Helsinki, Finland.
Clin Genet. 1996 Apr;49(4):196-9. doi: 10.1111/j.1399-0004.1996.tb03286.x.
We studied the origin of transferrin receptor (CD71) positive cells in blood from seven women pregnant with a male fetus in order to explore if fetal cells could be detected among them. We used a technique that allows direct chromosomal analysis by in situ hybridization on immunologically and morphologically classified cells. Enrichment was performed by magnetic activated cell sorting (miniMACS) using an anti-CD71 monoclonal antibody. The cells were immunophenotyped by alkaline phosphatase anti-alkaline phosphatase immunostaining with the same antibody. The origin of the immunophenotyped cells was studied by in situ hybridization using an X cosmid Y repeat chromosome specific probe cocktail. CD71 positive cells were found in six of the seven women at the range of 4 to 43 in respective samples. Over 90% of the CD71 positive cells were nucleated erythrocytes. None of the detected positive cells were shown to be fetal. Thus, the use of transferrin receptor antigen alone in combination with the miniMACS may not be sufficient for enrichment of fetal cells.
我们研究了7名怀有男性胎儿的孕妇血液中转铁蛋白受体（CD71）阳性细胞的来源，以探索其中是否能检测到胎儿细胞。我们采用了一种技术，可通过对免疫和形态学分类的细胞进行原位杂交来直接进行染色体分析。使用抗CD71单克隆抗体通过磁性激活细胞分选（miniMACS）进行富集。用同一抗体通过碱性磷酸酶抗碱性磷酸酶免疫染色对细胞进行免疫表型分析。使用X黏粒Y重复染色体特异性探针混合物通过原位杂交研究免疫表型细胞的来源。在7名女性中的6名各自的样本中发现了CD71阳性细胞，范围为4至43个。超过90%的CD71阳性细胞为有核红细胞。未检测到的阳性细胞显示为胎儿细胞。因此，单独使用转铁蛋白受体抗原并结合miniMACS可能不足以富集胎儿细胞。
