Donoghue A M
Germplasm and Gamete Physiology Laboratory, USDA, Beltsville, Maryland 20705, USA.
Poult Sci. 1996 Aug;75(8):1035-8. doi: 10.3382/ps.0751035.
Counting the holes in the perivitelline layer surrounding the germinal disc of freshly laid eggs has been used as a means of assessing sperm attachment, their ability to undergo an acrosome reaction, and penetration of the ovum, all necessary steps in the process of fertilization. The objective of this study was to compare the number of holes in the perivitelline layer of eggs after insemination with fresh turkey semen or with semen stored in vitro for 24 h. Hens (n = 40) were inseminated weekly with either 100 x 10(6) or 10 x 10(6) liable sperm within 90 min of collection or 24 h after in vitro storage at 5 C. A minimum of 20 eggs per treatment group were evaluated for the number of holes in the perivitelline layer. Fertility and hatchability were determined for all other eggs. The number of holes in eggs from hens inseminated with 100 x 10(6) sperm was higher (P < 0.05) for fresh (119 +/- 23 holes per egg) vs stored (75 +/- 5 holes per egg) semen treatments over the 10-wk study period. Holes observed in the perivitelline layer of eggs from hens inseminated with 100 x 10(6) sperm were more numerous than those inseminated with 10 x 10(6) sperm (P < 0.05). The number of holes observed for fresh and stored 10 x 10(6) groups were 6 +/- 3 and 7 +/- 4 holes per egg, respectively. Fertility was higher for the eggs from hens inseminated with 100 x 10(6) sperm than for those from hens inseminated with 10 x 10(6) sperm (P < 0.05). Hatchability of fertile eggs was higher for the 100 x 10(6) dose eggs than for eggs that received the low dose but did not differ between fresh and stored treatments. This report shows that the number of sperm that hydrolyze through the perivitelline layer is reduced after in vitro storage of semen.
计算刚产下的鸡蛋胚盘周围卵黄膜层上的孔洞数量，已被用作评估精子附着、顶体反应能力和卵子穿透能力的一种方法，这些都是受精过程中的必要步骤。本研究的目的是比较用新鲜火鸡精液或体外保存24小时的精液授精后，鸡蛋卵黄膜层上的孔洞数量。每周对40只母鸡在采集后90分钟内或在5℃体外保存24小时后，用100×10⁶或10×10⁶个活精子进行授精。每个处理组至少评估20个鸡蛋卵黄膜层上的孔洞数量。对所有其他鸡蛋测定受精率和孵化率。在为期10周的研究期间，用100×10⁶个精子授精的母鸡所产鸡蛋，新鲜精液处理组（每个鸡蛋119±23个孔洞）的卵黄膜层孔洞数量高于保存精液处理组（每个鸡蛋75±5个孔洞）（P<0.05）。用100×10⁶个精子授精的母鸡所产鸡蛋卵黄膜层上观察到的孔洞，比用10×10⁶个精子授精的母鸡所产鸡蛋上的孔洞更多（P<0.05）。新鲜和保存的1 × 10⁶组观察到的孔洞数量分别为每个鸡蛋6±3个和7±4个。用100×10⁶个精子授精的母鸡所产鸡蛋的受精率高于用10×10⁶个精子授精的母鸡所产鸡蛋（P<0.05）。100×10⁶剂量组的可育鸡蛋孵化率高于低剂量组，但新鲜和保存处理之间没有差异。本报告表明，精液体外保存后，穿过卵黄膜层水解的精子数量减少。
