Puissant C, Lennaouar M, Le Chalony C, Goubin G, Houdebine L M
Unité de Différenciation Cellulaire, Institut National de la Recherche Agronomique, Jouy en Josas, France.
Biochem Mol Biol Int. 1996 Mar;38(3):543-52.
The OZF gene identified recently is expressed in a few cell types and particularly in mammary cell but not in fibroblasts. This gene is expressed more abundantly in several mammary tumor cell lines than in normal cells. In the present work, OZF mRNA concentration was evaluated in rabbit and mouse mammary gland during the pregnancy-lactation-weaning cycle. The OZF mRNA concentration was at its highest level during the first part of pregnancy and decreased at parturition. It was maintained at its lowest level throughout lactation. Prolactin which induces mammary gland growth and milk synthesis in vivo did not stimulate OZF gene expression. In the Nb2 lymphoid cell line, prolactin, which is a compulsory growth factor, did not alter OZF mRNA concentration. These data suggest that OZF may be involved in some way in mammary gland growth. Moreover, OZF does not appear to be a key gene for the induction of cell multiplication. OZF gene expression might negatively control, to some extent, mammary cell differentiation.
最近鉴定出的OZF基因在少数几种细胞类型中表达，尤其在乳腺细胞中表达，但在成纤维细胞中不表达。该基因在几种乳腺肿瘤细胞系中的表达比在正常细胞中更为丰富。在本研究中，对兔和小鼠乳腺在妊娠-泌乳-断奶周期中的OZF mRNA浓度进行了评估。OZF mRNA浓度在妊娠初期达到最高水平，并在分娩时下降。在整个泌乳期，其浓度维持在最低水平。在体内诱导乳腺生长和乳汁合成的催乳素并未刺激OZF基因表达。在Nb2淋巴样细胞系中，作为一种必需生长因子的催乳素并未改变OZF mRNA浓度。这些数据表明，OZF可能在某种程度上参与乳腺生长。此外，OZF似乎不是诱导细胞增殖的关键基因。OZF基因表达可能在一定程度上对乳腺细胞分化起负调控作用。
