Niwa T, Koide N, Tsuji T, Imaoka S, Ishibashi F, Funae Y, Katagiri M
Division of Natural Science, Osaka Kyoiku University, Japan.
Res Commun Mol Pathol Pharmacol. 1996 Mar;91(3):372-8.
The maintenance of cytochrome P450s (P450s) and NADPH-cytochrome P450 reductase (P450 reductase) in the monolayer and spheroid cultures of hepatocytes from male rats was examined. The content of total P450 in monolayer culture decreased to almost none after 144 hr, whereas the level in spheroid culture remained within 6-13% of initial values during an incubation period of 144-192 hr. P450 2C11, a major P450 in male rat, in monolayer cells rapidly decreased in 144 hr, while the level in spheroid cells after 144 hr and 192 hr maintained 25% and 15%, respectively, of initial level. On the other hand, P450 2A1 and P450 2E1 in both monolayer and spheroid cells rapidly decreased. P450 reductase in both cells showed a gradual decline reaching a level of 43-44% of the initial level at 96 hr, and remained within 16-17% of the initial value during an incubation period of 192 hr. These results indicate that P450 2C11 in spheroid cells maintained more stable than in the monolayer cells, and that P450 reductase in both cultures declined only moderately, compared with P450s.
研究了雄性大鼠肝细胞单层培养和球状体培养中细胞色素P450（P450s）和NADPH-细胞色素P450还原酶（P450还原酶）的维持情况。单层培养中总P450含量在144小时后几乎降至零，而球状体培养中的水平在144 - 192小时的孵育期内保持在初始值的6 - 13%。雄性大鼠主要的P450——P450 2C11，在单层细胞中144小时内迅速下降，而球状体细胞中144小时和192小时后的水平分别维持在初始水平的25%和15%。另一方面，单层和球状体细胞中的P450 2A1和P450 2E1均迅速下降。两种细胞中的P450还原酶均呈逐渐下降趋势，在96小时时降至初始水平的43 - 44%，并在192小时的孵育期内维持在初始值的16 - 17%。这些结果表明，球状体细胞中的P450 2C11比单层细胞中的更稳定，并且与P450s相比，两种培养物中的P450还原酶仅适度下降。
