Yu M, Talbot P J
Laboratory of Neuroimmunovirology, Institut Armand-Frappier, Université Du Québec, Laval, Canada.
Adv Exp Med Biol. 1995;380:165-72. doi: 10.1007/978-1-4615-1899-0_27.
Neurotropic murine coronaviruses (MHV) provide an excellent animal model to study experimental modulation of the immune response to a viral pathogen with anti-idiotypic antibodies. It is known that among the various types of anti-idiotypic antibodies (anti-Id), those designated beta (beta) or internal image can molecularly mimic the antigen and induce biological activities such as anti-viral protection and neutralization. We have recently shown that polyclonal non-internal image anti-idiotypic antibodies of the gamma-type could induce protective anti-coronavirus immunity. In the present study, a polyclonal anti-Id (Ab2) was induced against a neutralizing murine monoclonal antibody (MAb1), designated 5B170.11. Mice immunized with this affinity-purified rabbit Ab2 alpha, a non-internal image antibody, were partially protected against lethal infection by the JHM strain of MHV. However, other polyclonal and monoclonal non-internal image Ab2 induced against another neutralizing Mab1, designated 4-11G.6, were not able to protect mice against lethal infection with the A59 strain of MHV. These results demonstrate that anti-viral protection by altering the idiotypic network with non-internal image-bearing anti-idiotype reagents can be achieved even with some anti-Id of the alpha-type.
嗜神经小鼠冠状病毒（MHV）为研究用抗独特型抗体对病毒病原体免疫反应进行实验性调节提供了一个出色的动物模型。已知在各种类型的抗独特型抗体（抗Id）中，那些被称为β（β）或内影像的抗体能够在分子水平上模拟抗原，并诱导诸如抗病毒保护和中和等生物学活性。我们最近表明，γ型多克隆非内影像抗独特型抗体能够诱导保护性抗冠状病毒免疫。在本研究中，针对一种名为5B170.11的中和小鼠单克隆抗体（MAb1）诱导产生了一种多克隆抗Id（Ab2）。用这种亲和纯化的兔Ab2α（一种非内影像抗体）免疫的小鼠，对MHV的JHM株致死性感染有部分保护作用。然而，针对另一种名为4-11G.6的中和Mab1诱导产生的其他多克隆和单克隆非内影像Ab2，却不能保护小鼠免受MHV A59株的致死性感染。这些结果表明，即使使用一些α型抗Id，通过用携带非内影像抗独特型试剂改变独特型网络也能够实现抗病毒保护。
