Sponar J, Votavová H
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Praha, Czech Republic.
J Biomol Struct Dyn. 1996 Jun;13(6):979-87. doi: 10.1080/07391102.1996.10508912.
Repetitive basic polypeptides containing lysine or arginine as every third amino acid were shown to cause DNA condensation at physiological salt concentration connected with selective DNA binding with respect to DNA composition and sequence. This selectivity is very similar to that existing in the case of histone H1 and other basic proteins and does not depend on polypeptide chain conformation. The effect of the minor groove binding drugs netropsin and distamycin was tested to elucidate the origin of the binding selectivity. The results suggest that the binding preferences are due to the variations in the conformation in various types of B-DNA that depend on DNA composition and sequence. The most important factor affecting the selectivity is probably the value of the negative electrostatic potential in the minor groove.
含有赖氨酸或精氨酸且每隔三个氨基酸出现一次的重复碱性多肽，在生理盐浓度下可导致DNA凝聚，这种凝聚与DNA组成和序列的选择性DNA结合有关。这种选择性与组蛋白H1和其他碱性蛋白的情况非常相似，且不依赖于多肽链构象。测试了小沟结合药物纺锤菌素和偏端霉素的作用，以阐明结合选择性的起源。结果表明，结合偏好是由于不同类型B-DNA构象的变化，这些变化取决于DNA组成和序列。影响选择性的最重要因素可能是小沟中负静电势的值。
