Pandey S C
Department of Psychiatry (M/C912), College of Medicine, University of Illinois at Chicago 60612, USA.
Neurosci Lett. 1996 Jul 12;212(2):127-30. doi: 10.1016/0304-3940(96)12817-2.
The effect of chronic nicotine treatment on postreceptor sites of the phosphoinositide (PI) signaling system (i.e. phospholipase C (PLC) isozymes, Gq/11 protein) in the rat cortex was investigated. The expression of PLC isozymes (beta 1, gamma 1, and delta 1) and the alpha subunit of Gq/11 protein in the cortices of rats treated with saline or nicotine was determined by the Western blot technique using specific monoclonal antibodies. It was observed that chronic nicotine treatment (10 days) significantly increased the immunolabeling of the total PLC-beta 1, which was due to an increase in the immunolabeling of both the membranal and the cytosolic PLC-beta 1 isozyme in the rat cortex. On the other hand, the immunolabeling of total, membranal, and cytosolic PLC-delta 1 and -gamma 1 isozymes in the rat cortex was not significantly altered by chronic nicotine treatment. In addition, Western blot analysis also demonstrated that chronic nicotine treatment has no significant effect on the immunolabeling of the alpha subunit of Gq/11 protein in the rat cortex. Thus, these findings suggest that an increase in the expression of the PLC-beta 1 isozyme may be involved in the neuroadaptational mechanisms associated with tobacco addiction.
研究了慢性尼古丁处理对大鼠皮质中磷酸肌醇（PI）信号系统的受体后位点（即磷脂酶C（PLC）同工酶、Gq/11蛋白）的影响。使用特异性单克隆抗体，通过蛋白质免疫印迹技术测定用生理盐水或尼古丁处理的大鼠皮质中PLC同工酶（β1、γ1和δ1）和Gq/11蛋白α亚基的表达。观察到慢性尼古丁处理（10天）显著增加了总PLC-β1的免疫标记，这是由于大鼠皮质中膜结合型和胞质型PLC-β1同工酶的免疫标记均增加。另一方面，慢性尼古丁处理未显著改变大鼠皮质中总、膜结合型和胞质型PLC-δ1和-γ1同工酶的免疫标记。此外，蛋白质免疫印迹分析还表明，慢性尼古丁处理对大鼠皮质中Gq/11蛋白α亚基的免疫标记没有显著影响。因此，这些发现表明PLC-β1同工酶表达的增加可能参与了与烟草成瘾相关的神经适应性机制。
