Kahari L, Jimenez S A
Division of Rheumatology, Department of Medicine, Thomas Jefferson University, Philadelphia, PA, USA.
J Rheumatol. 1996 Mar;23(3):482-6.
To compare the expression of the genes encoding transforming growth factor-beta1 (TCF-beta1) and several extracellular matrix proteins between fascial and dermal fibroblasts from 3 patients with diffuse fasciitis with eosinophilia (DFE) of recent onset.
Fibroblasts were separately cultured from the fascia and dermis from each patient. Collagen production and mRNA levels for fibronectin, alpha1(I) procollagen, alpha1(III) procollagen. and the 3 chains of type VI collagen were compared between fascial and dermal fibroblasts from the same patient using biosynthetic studies with 14C-proline and Northern and dot blot hybridizations with specific cDNA. The expression of the gene encoding TGF-beta1 was also examined in these cultures by Northern hybridizations with human TGF-beta1 cDNA.
Fascial fibroblasts displayed 1.75 to 4.6-fold greater collagen biosynthesis, 3.4 to 8.5-fold elevation of the steady state levels of fibronectin mRNA, up to 3.9-fold elevation of the steady state mRNA levels for alpha1(I), alpha1(III) and alpha3(VI) collagens, and a marked increase in TGF-beta1 mRNA levels compared to fibroblasts from the adjacent dermis from the same individuals.
The expression of genes encoding several extracellular matrix proteins is increased in fascial fibroblasts from patients with diffuse fasciitis compared to fibroblasts from the adjacent dermis of the same individuals. The elevation of TGF-beta1 mRNA in the fascial cells indicates that this growth factor may play an important role in the pathogenesis of fibrosis in DFE.
比较3例近期发病的嗜酸性粒细胞增多性弥漫性筋膜炎（DFE）患者筋膜成纤维细胞和真皮成纤维细胞中转化生长因子-β1（TGF-β1）及几种细胞外基质蛋白编码基因的表达。
从每位患者的筋膜和真皮中分别培养成纤维细胞。使用含14C-脯氨酸的生物合成研究以及与特异性cDNA的Northern杂交和斑点杂交，比较同一患者筋膜成纤维细胞和真皮成纤维细胞之间的胶原蛋白生成以及纤连蛋白、α1（I）前胶原、α1（III）前胶原和VI型胶原3条链的mRNA水平。还通过与人TGF-β1 cDNA的Northern杂交检测这些培养物中TGF-β1编码基因的表达。
与同一患者相邻真皮的成纤维细胞相比，筋膜成纤维细胞的胶原蛋白生物合成增加了1.75至4.6倍，纤连蛋白mRNA的稳态水平升高了3.4至8.5倍，α1（I）、α1（III）和α3（VI）胶原的稳态mRNA水平升高了3.9倍，TGF-β1 mRNA水平显著增加。
与同一患者相邻真皮的成纤维细胞相比，弥漫性筋膜炎患者筋膜成纤维细胞中几种细胞外基质蛋白编码基因的表达增加。筋膜细胞中TGF-β1 mRNA的升高表明该生长因子可能在DFE的纤维化发病机制中起重要作用。
