Dominguez H D, Lopez M F, Chotro M G, Molina J C
Instituto de Investigacion Medica Mercedes y Martin Ferreyra, Cordoba, Argentina.
Neurobiol Learn Mem. 1996 Mar;65(2):103-12. doi: 10.1006/nlme.1996.0012.
Rat fetuses proximal to birth process alcohol-derived cues when the drug is directly delivered into the amniotic fluid. Prior evidence indicates that chemosensory sensation is detected during gestational Day 17 (GD17). In the present study Wistar-derived pregnant females received 0, 1, or 2 g/kg/day of alcohol (intragastric intubation) during GDs 17-20. Prenatal treatment failed to affect different maternal-fetal and perinatal physical parameters, e.g., placenta weight, umbilical cord length, offspring's body weights, weights and/or size of the olfactory bulbs, cerebral hemispheres, and cerebellum. Alcohol chemosensory responsiveness assessed in a perinatal motor activity test, indicated that pups pretreated with the 1 and 2 g/kg alcohol dose exhibit significant decrements in their activity rate when alcohol odor is presented in the test chamber. Alcohol concentrations in maternal and fetal blood and in the amniotic fluid were also recorded through head-space chromatography 1 h after females received the last intubation procedure (GD20) with the 1 or 2 g/kg alcohol doses. Dose-dependent alcohol concentrations across the different sites of assessment were recorded. As indicated by previous studies, even the alcohol level in the amniotic fluid attained with the 1 g/kg alcohol dose is above threshold values in terms of allowing fetal chemosensory stimulation with alcohol-derived cues. The results suggest that maternal ethanol intoxication during the last days of pregnancy leads to fetal exposure to alcohol's sensory attributes and that this experience subsequently modifies responsiveness to these cues.
当药物直接注入羊水时，临近分娩过程的大鼠胎儿会感知酒精衍生的线索。先前的证据表明，在妊娠第17天（GD17）可检测到化学感觉。在本研究中，Wistar品系的怀孕雌性大鼠在GD17 - 20期间接受0、1或2 g/kg/天的酒精（胃内插管）。产前治疗未能影响不同的母胎和围产期身体参数，例如胎盘重量、脐带长度、后代体重、嗅球、大脑半球和小脑的重量和/或大小。在围产期运动活动测试中评估的酒精化学感觉反应性表明，用1和2 g/kg酒精剂量预处理的幼崽在测试室中出现酒精气味时，其活动率显著下降。在雌性大鼠接受1或2 g/kg酒精剂量的最后一次插管程序（GD20）1小时后，还通过顶空气相色谱法记录了母血、胎儿血和羊水中的酒精浓度。记录了不同评估部位的剂量依赖性酒精浓度。如先前研究所示，即使1 g/kg酒精剂量达到的羊水中酒精水平，就允许胎儿通过酒精衍生线索进行化学感觉刺激而言，也高于阈值。结果表明，妊娠最后几天母体乙醇中毒会导致胎儿接触酒精的感官属性，并且这种经历随后会改变对这些线索的反应性。
