Cataltepe O, Towfighi J, Vannucci R C
Department of Pediatrics, The Pennsylvania State University College of Medicine, The Milton S. Hershey Medical Center, Hershey, PA 17033-0850, USA.
Brain Res. 1996 Feb 19;709(2):326-30. doi: 10.1016/0006-8993(95)01437-3.
Cerebrospinal fluid (CSF) concentrations of glutamate and gamma- aminobutyric acid (GABA), as estimates of levels in the extracellular compartment of brain, were determined in 7-day postnatal rats at the terminus of hypoxia-ischemia and during status epilepticus, induced with bicuculline, at 2 and 24 h of recovery. Hypoxia-ischemia was associated with increased CSF glutamate, which was not increased further during status epilepticus. In contrast, CSF GABA was increased by hypoxia-ischemia as well as by status epilepticus during recovery. CSF glutamate/GABA ratios in rat pups subjected to status epilepticus with or without prior hypoxia-ischemia were lower than control animals during recovery. The lack of any significant increase in glutamate or in the glutamate/GABA ratio during status epilepticus would preclude any neuronal injury from occurring in those immature rats sustaining seizures alone or any accentuation of brain damage in those animals subjected to prior cerebral hypoxia-ischemia.
在出生后7天的大鼠中，于缺氧缺血结束时以及在恢复2小时和24小时时，通过荷包牡丹碱诱导癫痫持续状态，测定了脑脊液（CSF）中谷氨酸和γ-氨基丁酸（GABA）的浓度，以此作为脑细胞外间隙水平的估计值。缺氧缺血与脑脊液谷氨酸水平升高有关，而在癫痫持续状态期间并未进一步升高。相比之下，缺氧缺血以及恢复期间的癫痫持续状态均使脑脊液GABA水平升高。在恢复期间，经历癫痫持续状态（无论是否有先前的缺氧缺血）的幼鼠脑脊液谷氨酸/ GABA比值低于对照动物。在癫痫持续状态期间谷氨酸或谷氨酸/ GABA比值没有任何显著增加，这将排除在仅发生癫痫发作的那些未成熟大鼠中发生任何神经元损伤，或排除在先前经历脑缺氧缺血的那些动物中脑损伤的任何加重情况。
