Ellison D A, Silverman J F, Strausbach P S, Joshi V V
Department of Pathology and Laboratory Medicine, East Carolina University School of Medicine, Greenville, NC 27858, USA.
Diagn Cytopathol. 1996 Feb;14(1):51-5. doi: 10.1002/(SICI)1097-0339(199602)14:1<51::AID-DC10>3.0.CO;2-C.
We describe an unusual case of chondroblastic osteosarcoma of the skull in an 11-yr-old girl in whom a preoperative diagnosis was made by fine-needle aspiration (FNA) biopsy, followed by histologic confirmation of tissue biopsy and the surgically resected specimen. FNA cytology revealed pleomorphic oval cells with prominent nucleoli along with spindle cells, tumor giant cells, and a chondromyxoid background. The cell block of the aspirated material showed osteoid associated with the malignant cells. Immunocytochemical stains revealed S-100 and vimentin positivity; actin, myoglobin, and cytokeratin stains were negative. Electron microscopy revealed neoplastic cells with chondrocytic differentiation. This case demonstrates the value of FNA biopsy combined with immunocytochemical and ultrastructural studies performed on the aspirated material in diagnosing osteosarcoma from an unusual location such as the base of the skull.
我们描述了一名11岁女孩发生于颅骨的软骨母细胞性骨肉瘤的罕见病例，该病例通过细针穿刺（FNA）活检进行了术前诊断，随后通过组织活检和手术切除标本进行了组织学确认。FNA细胞学检查显示多形性椭圆形细胞，伴有明显核仁，同时还有梭形细胞、肿瘤巨细胞以及软骨黏液样背景。吸出物的细胞块显示有与恶性细胞相关的类骨质。免疫细胞化学染色显示S-100和波形蛋白呈阳性；肌动蛋白、肌红蛋白和细胞角蛋白染色呈阴性。电子显微镜检查显示肿瘤细胞具有软骨细胞分化。该病例证明了FNA活检结合对吸出物进行免疫细胞化学和超微结构研究在诊断来自不寻常部位（如颅底）的骨肉瘤中的价值。
