Pujana M A, Volpini V, Estivill X
Molecular Genetics Department, Cancer Research Institute (IRO), L'Hospitalet de Llobregat, Barcelona, Spain.
Nucleic Acids Res. 1996 Sep 15;24(18):3651-2. doi: 10.1093/nar/24.18.3651.
We report here an Alu-(CAG/GTC)n PCR method for the cloning of STSs with (CAG/GTC)n sequences. We have applied this method to genomic DNA of a somatic cell hybrid containing human chromosome 12 where linkage has been found for a known familiar dominant ataxia (SCA2), which is thought to be due to a (CAG/GTC)n expansion. We have isolated several clones containing (CAG/GTC)n sequences, which include previously identified sequences that map to chromosome 12. This method could be a new PCR approach for the cloning of repeats based on their proximity to Alu sequences.
我们在此报告一种用于克隆具有（CAG/GTC）n序列的序列标签位点（STS）的Alu-（CAG/GTC）n聚合酶链反应（PCR）方法。我们已将此方法应用于含有人类12号染色体的体细胞杂种的基因组DNA，在该杂种中已发现与一种已知的家族性显性共济失调（脊髓小脑共济失调2型，SCA2）存在连锁关系，该病被认为是由（CAG/GTC）n扩增所致。我们已分离出几个含有（CAG/GTC）n序列的克隆，其中包括先前鉴定出的定位于12号染色体的序列。该方法可能是一种基于与Alu序列的接近程度来克隆重复序列的新型PCR方法。
