Kato T, Kimizuka R, Okuda K
Department of Microbiology, Tokyo Dental College, Chiba, Japan.
FEMS Microbiol Lett. 1996 Oct 1;143(2-3):217-21. doi: 10.1111/j.1574-6968.1996.tb08483.x.
Periodontopathic Actinobacillus actinomyceremcomitans produces hemolysin and other leukotoxins. In the present study, two distinct clones which lysed horse erythrocytes were isolated by screening genomic DNA libraries of A. actinomycetemcomitans ATCC 43718 on blood agar plates. DNA hybridization analysis indicates that there were two distinct hemolytic genes present. Sonicated extracts from both Escherichia coli clones possessed hemolytic activities on horse, sheep and human erythrocytes, but not those of rabbit. Rabbit antiserum to A. actinomycetemcomitans ATCC 43718 whole cells inhibited the hemolytic activities of these clones.
牙周病原菌伴放线放线杆菌可产生溶血素和其他白细胞毒素。在本研究中，通过在血琼脂平板上筛选伴放线放线杆菌ATCC 43718的基因组DNA文库，分离出了两个能裂解马红细胞的不同克隆。DNA杂交分析表明存在两个不同的溶血基因。来自这两个大肠杆菌克隆的超声破碎提取物对马、羊和人的红细胞具有溶血活性，但对兔红细胞则无此活性。针对伴放线放线杆菌ATCC 43718全细胞的兔抗血清可抑制这些克隆的溶血活性。
