一种测定大鼠肝脏微粒体3-羟基-3-甲基戊二酰辅酶A还原酶活性的新方法及其在膳食胆固醇对该酶影响研究中的应用。
A new method for assaying rat liver microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity and its application in a study of the effect of dietary cholesterol on this effect of dietary cholesterol on this enzyme.
作者信息
Baqir Y A, Booth R
出版信息
Biochem J. 1977 Jun 15;164(3):501-8. doi: 10.1042/bj1640501.
Abstract
A new method suitable for measuring rat liver 3-hydroxy-3-methylglutaryl-CoA reductase activity is described and its advantages over methods previously available are discussed. An accurate time course was measured for the inhibition of liver microsomal 3-hydroxy-3-methylglutaryl-CoA reductase activity by dietary cholesterol; this enzyme was affected 1 1/4 h after the rats began to consume a cholesterol-rich diet. In this experiment there was no correlation between concentrations of microsomal cholesterol ester and the activity of 3-hydroxy-3-methylglutary-CoA reductase.
摘要
本文描述了一种适用于测量大鼠肝脏3-羟基-3-甲基戊二酰辅酶A还原酶活性的新方法，并讨论了该方法相对于先前可用方法的优势。测定了膳食胆固醇对肝脏微粒体3-羟基-3-甲基戊二酰辅酶A还原酶活性抑制作用的准确时间进程；大鼠开始食用富含胆固醇的饮食后1 1/4小时，该酶受到影响。在本实验中，微粒体胆固醇酯浓度与3-羟基-3-甲基戊二酰辅酶A还原酶活性之间没有相关性。
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A new method for assaying rat liver microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity and its application in a study of the effect of dietary cholesterol on this effect of dietary cholesterol on this enzyme.一种测定大鼠肝脏微粒体3-羟基-3-甲基戊二酰辅酶A还原酶活性的新方法及其在膳食胆固醇对该酶影响研究中的应用。
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