Oguni M, Tamura H, Kato K, Setogawa T
Department of Ophthalmology, Shimane Medical University, Izumo, Japan.
Histol Histopathol. 1996 Jul;11(3):695-702.
Recently ultraviolet light (UV) reaching the Earth's surface has been gradually increasing in amounts by the destruction of the ozone layers. Large parts of UV are absorbed in the cornea and lens, and only a few amounts reached the retina; however, the effect on the retina is not fully elucidated. 38 rats were irradiated 0.5-5.0 J/cm2 UV from 6 to 50 times every 24 hours, and immunohistochemically and immunochemically for superoxide dismutases (SOD). Morphologically, the destruction of rod outer segments (ROS) and dissociation of cell membranes between the pigment epithelial cells (PE) were already observed by 6 times 0.5 J/cm2 UV irradiations. As the doses of UV increased, heterochromatins and lipid droplets increased in the PE. In normal retina, Cu/Zn SOD were mainly distributed from the inner limiting membrane (ILM) to the ganglion cell layer, and the PE; however, after 6 times 0.5 J/cm2 UV irradiations, the distribution became widened from inner to outer plexiform layer (OPL). At that time, the concentrations of Cu/Zn and Mn SOD increased in the retina. The present study reveals that the morphological damage caused by UV irradiation is observed in the ROS and PE, where no immunoreactivities could be detected to Cu/Zn and Mn SOD. However, morphological damage was not from the ILM to OPL, where the immunoreactivities to both Cu/Zn and Mn SOD were observed.
最近，由于臭氧层遭到破坏，到达地球表面的紫外线（UV）量在逐渐增加。大部分紫外线被角膜和晶状体吸收，只有少量能到达视网膜；然而，其对视网膜的影响尚未完全阐明。对38只大鼠每24小时进行6至50次0.5 - 5.0 J/cm²的紫外线照射，并对超氧化物歧化酶（SOD）进行免疫组织化学和免疫化学检测。形态学上，在0.5 J/cm²紫外线照射6次后，已观察到视杆细胞外段（ROS）的破坏以及色素上皮细胞（PE）之间细胞膜的解离。随着紫外线剂量增加，PE中的异染色质和脂滴增多。在正常视网膜中，铜/锌超氧化物歧化酶（Cu/Zn SOD）主要分布于内界膜（ILM）至神经节细胞层以及PE；然而，在0.5 J/cm²紫外线照射6次后，其分布从内网状层扩展至外网状层（OPL）。此时，视网膜中Cu/Zn和锰超氧化物歧化酶（Mn SOD）的浓度增加。本研究表明，紫外线照射引起的形态学损伤在ROS和PE中可见，在这些部位检测不到Cu/Zn和Mn SOD的免疫反应性。然而，从ILM到OPL未出现形态学损伤，在这些部位可观察到Cu/Zn和Mn SOD的免疫反应性。
