Bonnet F, Vernotte C, Briantais J M, Etienne A L
Biochim Biophys Acta. 1977 Jul 7;461(1):151-8. doi: 10.1016/0005-2728(77)90077-9.
The kinetics of chlorophyll fluorescence at 77K were studied in Chlorella cells and spinach chloroplasts. During a first illumination, the rise is polyphasic with at least three phases. The slowest one is irreversible and corresponds to the cytochrome oxidation. The dark regeneration of half the variable fluorescence is biphasic, the fast phase being inhibited by 3-(3,4-dichlorophenyl)-1, 1-dimethylurea (DCMU) both in Chlorella and chloroplasts. The fluorescence rise during a second illumination is still biphasic. Carbonyl cyanide m-chlorophenylhydrazone (CCCP) slows down the fluorescence rise in Chlorella but has no effect on the dark regeneration. It does not affect the fluorescence of chloroplasts. Ferricyanide which oxidizes cytochrome beta-559 at room temperature produces a quenching of the variable fluorescence and an acceleration of the fluorescence rise during the first illumination. Our results fit the idea of the heterogeneity of the Photosystem II centers at low temperature.
在小球藻细胞和菠菜叶绿体中研究了77K时叶绿素荧光的动力学。在第一次光照期间，荧光上升是多相的，至少有三个阶段。最慢的阶段是不可逆的，与细胞色素氧化相对应。可变荧光一半的暗再生是双相的，快速阶段在小球藻和叶绿体中均受到3-(3,4-二氯苯基)-1,1-二甲基脲（DCMU）的抑制。第二次光照期间的荧光上升仍是双相的。羰基氰化物间氯苯腙（CCCP）减缓了小球藻中的荧光上升，但对暗再生没有影响。它不影响叶绿体的荧光。在室温下氧化细胞色素β-559的铁氰化物会导致可变荧光猝灭，并在第一次光照期间加速荧光上升。我们的结果符合低温下光系统II中心异质性的观点。
