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羰基氰化物间氯苯腙对小球藻中光系统II中心的光灭活作用

Photo-inactivation of system II centers by carbonyl cyanide m-chlorophenylhydrazone in Chlorella pyrenoidosa.

作者信息

Lemasson C, Etienne A L

出版信息

Biochim Biophys Acta. 1975 Nov 11;408(2):135-42. doi: 10.1016/0005-2728(75)90005-5.

DOI:10.1016/0005-2728(75)90005-5
PMID:1191652
Abstract

In the presence of a high concentration of carbonyl cyanide m-chlorophenylhydrazone (CCCP) (4-10(-6) M), the S2 and S3 dark decays are accelerated and become biphasic with a first half-time of 0.6 s. The first fast phase of the decays does not correspond to a simple reduction of S2, S3 back to S0, S1 (i.e. to an acceleration of the deactivation reaction), but to a decrease in the number of oxygen-evolving System II centers. This photo-inactivation produced by CCCP is rapidly reversible in the dark.

摘要

在存在高浓度羰基氰化物间氯苯腙(CCCP)(4×10⁻⁶ M)的情况下,S2和S3的暗衰减加速并变为双相,首个半衰期为0.6秒。衰减的首个快速阶段并非对应于S2、S3简单地还原为S0、S1(即失活反应的加速),而是对应于放氧系统II中心数量的减少。CCCP产生的这种光失活在黑暗中可迅速逆转。

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