Vaughn J P, Cirisano F D, Huper G, Berchuck A, Futreal P A, Marks J R, Iglehart J D
Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA.
Cancer Res. 1996 Oct 15;56(20):4590-4.
Identifying the conditions and kinetics of the induction of BRCA2 gene expression may implicate roles for the function of the tumor suppressor gene. In this study, expression of BRCA2 mRNA is shown to be regulated by the cell cycle and associated with proliferation in normal and tumor-derived breast epithelial cells. Cells arrested in G(0) or early G1 contained low levels of BRCA2 mRNA. After release into a proliferating state, cells produced maximum levels of BRCA2 mRNA in late G1 and the S-phase. Similar cell cycle control of BRCA2 was observed in fractions of exponentially growing cells isolated by centrifugal elutriation. Expression of BRCA2 was shown to be independent of bulk DNA synthesis. In addition, the kinetics of BRCA2 mRNA up-regulation appeared to be similar to those of BRCA1, suggesting that the two genes could be commonly controlled. These results imply that these two tumor suppressor genes are utilized during growth and may have a protective role in cellular proliferation.
确定BRCA2基因表达诱导的条件和动力学可能暗示该肿瘤抑制基因功能的作用。在本研究中，BRCA2 mRNA的表达显示受细胞周期调控，并与正常和肿瘤来源的乳腺上皮细胞增殖相关。停滞于G(0)期或G1早期的细胞含有低水平的BRCA2 mRNA。释放到增殖状态后，细胞在G1晚期和S期产生最高水平的BRCA2 mRNA。通过离心淘析分离的指数生长细胞组分中观察到类似的BRCA2细胞周期调控。BRCA2的表达显示与大量DNA合成无关。此外，BRCA2 mRNA上调的动力学似乎与BRCA1相似，表明这两个基因可能受到共同调控。这些结果暗示这两个肿瘤抑制基因在生长过程中被利用，并且可能在细胞增殖中具有保护作用。
