Tamburro A M, Guantieri V, Daga-Gordini D, Abatangelo G
Biochim Biophys Acta. 1977 Jun 24;492(2):370-6. doi: 10.1016/0005-2795(77)90088-5.
The alpha-elastin, the soluble product obtained from elastin by oxalic acid hydrolysis, has been studied from a conformational point of view in different conditions using circular dichroism spectroscopy. The spectral variations of the alpha-elastin dissolved in water and in different organic solvents/water mixtures have been examined in order to establish the effect of the solvents on the structural properties of the protein. The effect of calcium ions has also been studied on alpha-elastin dissolved either in water or in organic solvents. Finally, the effect of pH and temperature on the conformational properties of the alpha-elastin has been investigated.
α-弹性蛋白是通过草酸水解从弹性蛋白中获得的可溶性产物，已使用圆二色光谱法在不同条件下从构象角度对其进行了研究。研究了溶解在水中以及不同有机溶剂/水混合物中的α-弹性蛋白的光谱变化，以确定溶剂对蛋白质结构性质的影响。还研究了钙离子对溶解在水或有机溶剂中的α-弹性蛋白的影响。最后，研究了pH值和温度对α-弹性蛋白构象性质的影响。
