Bonwick G A, Cresswell J E, Tyreman A L, Baugh P J, Williams J H, Smith C J, Armitage R, Davies D H
MRIC Immunology Group, Athrofa Addsyg Uwch Gogledd Ddwyrain Cymru (NEWI), Clwyd, UK.
J Immunol Methods. 1996 Sep 27;196(2):163-73. doi: 10.1016/0022-1759(96)00098-1.
Monoclonal murine anti-pesticide antibodies were produced by in vitro immunisation (IVI) of cultured splenocytes with the pesticides sulcofuron and flucofuron. The majority of both anti-flucofuron and anti-sulcofuron antibodies obtained were of the IgM isotype, rather than IgG. When used in an indirect enzyme-linked immunosorbent assay (ELISA), the antibodies bound to plate coating antigens which incorporated haptens that mimicked moieties present within the immunising pesticide. The antibodies exhibited a high degree of specificity, with the degree of cross-reactivity related to the structural similarity between the hapten present in the plate coating antigen and the moieties present within the immunising pesticide. These results indicated that antibodies specific to both sulcofuron and flucofuron had been produced by IVI. Synthesis of both hapten analogues and immunogens as required for methods based on in vivo immunisation was avoided, whilst antibody production was also comparatively more rapid than traditional methods and minimised animal discomfort.
通过用农药磺胺磺隆和氟磺隆对培养的脾细胞进行体外免疫（IVI），产生了单克隆鼠抗农药抗体。所获得的大多数抗氟磺隆和抗磺胺磺隆抗体均为IgM同种型，而非IgG。当用于间接酶联免疫吸附测定（ELISA）时，这些抗体与包被在酶标板上的抗原结合，这些抗原掺入了模拟免疫农药中存在部分的半抗原。这些抗体表现出高度的特异性，交叉反应程度与酶标板包被抗原中存在的半抗原和免疫农药中存在的部分之间的结构相似性有关。这些结果表明，通过体外免疫产生了对磺胺磺隆和氟磺隆均具有特异性的抗体。避免了基于体内免疫的方法所需的半抗原类似物和免疫原的合成，同时抗体产生也比传统方法相对更快，并将动物不适降至最低。
