水溶性聚合物对聚集的亚铁血红素复合物和原卟啉IX的解离作用

Dissociation of aggregated ferroheme complexes and protoporphyrin IX by water-soluble polymers.

作者信息

Nishide H, Mihayashi K, Tsuchida E

出版信息

Biochim Biophys Acta. 1977 Jun 23;498(1):208-14. doi: 10.1016/0304-4165(77)90100-3.

Abstract

The ferroheme-pyridine complex, ferroheme and protoporphyrin IX form the aggregates by the hydrophobic interaction in aqueous solutions. We found by spectrophotometric and fluorometric measurements that the aggregates dissociated into the monomers by the addition of water-soluble polymers, such as, poly(ethyleneoxide), poly(vinylalcohol), poly(vinylpyrrolidone) and poly(styrene sulfonate). The dissociation by the polymers proceeded as their hydrophobicities increased. The aggregated ferroheme was effectively dissociated by the copolymers of 4-vinylpyridine which were water-soluble polymer-ligands.

摘要

亚铁血红素 - 吡啶配合物、亚铁血红素和原卟啉IX在水溶液中通过疏水相互作用形成聚集体。我们通过分光光度法和荧光测量发现，通过添加水溶性聚合物，如聚环氧乙烷、聚乙烯醇、聚乙烯吡咯烷酮和聚苯乙烯磺酸盐，聚集体会解离成单体。聚合物引起的解离随着其疏水性的增加而进行。聚集的亚铁血红素能被作为水溶性聚合物配体的4-乙烯基吡啶共聚物有效地解离。