Krepela E, Procházka J, Mynaríková H, Kárová B, Cermák J, Roubková H
Department of Molecular and Cellular Pneumology, Medical Faculty Hospital Bulovka, Prague, Czech Republic.
Neoplasma. 1996;43(3):171-8.
In the present work we studied the levels of activities of dipeptidyl-peptidase I (or cathepsin C, DPP-I) and dipeptidyl-peptidase II (DPP-II) and examined their isoelectric focusing profiles in matched pairs of human squamous cell lung carcinoma (SQCLC) and the lung from surgically treated patients (n = 33). The mean specific activities of DPP-I and DPP-II were higher in SQCLC (Stages I and II) than in the lung, but only the activity of DPP-II in Stage I SQCLC was significantly higher compared to the lung. The activities of both enzymes were higher in the tumor than in the lung in 10 of 20 Stage I SQCLC patients, but only in 3 of 13 Stage II SQCLC patients. The specific activities of DPP-I and DPP-II in the lungs showed a good correlation while the correlation of both enzyme activities in SQCLCs was poor. We observed only a small and mutually comparable activation of DPP-I in extracts from SQCLCs and from the lungs by dithiothreitol. The isoelectric focusing profile of several DPP-II forms in SQCLCs and the lungs was similar and the single major DPP-II isoform revealed in the tumors and lungs showed a pIapp of 5.3-5.2. The isoelectric focusing profile of DPP-I showed multiple enzyme forms in SQCLCs (pIapp 6.3-4.5) as well as in the lungs (pIapp 6.4-4.8). In SQCLCs, as well as in the lungs, the activities of the DPP-I forms with pIapp values < or = 5.6 were shifted by neuraminidase treatment to the site of the major DPP-I isoform with pIapp of about 6.0 and the zymograms then showed an another DPP-I with pIapp of 5.7, which was less discernible in the lung. In some patients, the DPP-I forms with pIapp values < or = 5.6 from SQCLC retained a greater percentage of activity distribution than did the DPP-I pIapp-counterparts from the lung.
在本研究中，我们检测了33例接受手术治疗的人类肺鳞状细胞癌（SQCLC）患者及其肺部组织中，二肽基肽酶I（或组织蛋白酶C，DPP-I）和二肽基肽酶II（DPP-II）的活性水平，并研究了它们的等电聚焦图谱。SQCLC（I期和II期）中DPP-I和DPP-II的平均比活性高于肺部组织，但仅I期SQCLC中DPP-II的活性显著高于肺部组织。20例I期SQCLC患者中，有10例肿瘤中两种酶的活性高于肺部组织，但13例II期SQCLC患者中只有3例如此。肺部组织中DPP-I和DPP-II的比活性具有良好的相关性，而SQCLC中两种酶活性的相关性较差。我们发现，二硫苏糖醇对SQCLC和肺部组织提取物中DPP-I的激活作用较小且相互可比。SQCLC和肺部组织中几种DPP-II形式的等电聚焦图谱相似，肿瘤和肺部组织中显示的单一主要DPP-II同工型的表观pI为5.3 - 5.2。DPP-I的等电聚焦图谱显示，SQCLC（表观pI 6.3 - 4.5）和肺部组织（表观pI 6.4 - 4.8）中均有多种酶形式。在SQCLC和肺部组织中，表观pI值≤5.6的DPP-I形式经神经氨酸酶处理后，其活性迁移至表观pI约为6.0的主要DPP-I同工型位置，酶谱图随后显示出另一种表观pI为5.7的DPP-I，这在肺部组织中较难分辨。在一些患者中，SQCLC中表观pI值≤5.6的DPP-I形式比肺部组织中相应表观pI的DPP-I形式保留了更高比例的活性分布。
