Bhavanandan V P, Breindel D, Davidson E A
Biochim Biophys Acta. 1977 Jun 23;498(1):250-4. doi: 10.1016/0304-4165(77)90105-2.
The treatment of chondroitin sulfate isolated from cultured B16 mouse melanoma cells with 0.04 M HCl at 100 degrees C for 90 min released up to 45% of O-sulfate residues as free inorganic sulfate. In addition to the release of inorganic sulfate, extensive degradation of this polysaccharide as well as of cartilage chondroitin sulfate, pig rib cartilage proteoglycan, heparin and hyaluronic acid was also evident under these conditions. The above hydrolysis conditions are used for characterizing 35S-labeled heparan sulfates synthesized by cultured cells and to calculate ratio of N- and O-sulfates in these molecules. Our results suggest that caution is necessary in interpreting the results of mild acid hydrolysis of glycosaminoglycans.
从培养的B16小鼠黑色素瘤细胞中分离出的硫酸软骨素，在100℃下用0.04 M盐酸处理90分钟，可释放高达45%的O-硫酸酯残基作为游离无机硫酸盐。除了释放无机硫酸盐外，在这些条件下，这种多糖以及软骨硫酸软骨素、猪肋软骨蛋白聚糖、肝素和透明质酸也出现了广泛降解。上述水解条件用于表征培养细胞合成的35S标记的硫酸乙酰肝素，并计算这些分子中N-硫酸盐和O-硫酸盐的比例。我们的结果表明，在解释糖胺聚糖的温和酸水解结果时需要谨慎。
