寡核苷酸与RecA蛋白单链DNA结合位点的相互作用。

Interaction of oligonucleotides with a single stranded DNA binding site of RecA protein.

作者信息

Nakano S, Sasaki M, Sugimoto N

机构信息

Department of Chemistry, Faculty of Science, Konan University, Kobe, Japan.

出版信息

Nucleic Acids Symp Ser. 1995(34):61-2.

PMID:8841552

Abstract

Model peptide Ile-Arg-Met-Lys-Ile-Gly-Val-Met-Phe-Gly-Asn-Pro-Glu-Thr- Thr-Thr-Gly-Gly-Asn-Ala-Leu-Lys-Phe-Tyr for a single stranded DNA (ssDNA) binding site of RecA protein has been synthesized and its interaction with some ssDNAs and ssRNAs has been investigated. Circular dichroism spectra showed that conformational change of the peptide by the addition of a ssDNA dACCTAGTC was much faster than that by the addition of a ssRNA rACCUAGUC. The addition of a neutral DNA or a 2'-5' linked oligonucleotide did not lead to the conformational change of the peptide.

摘要

已合成了用于RecA蛋白单链DNA（ssDNA）结合位点的模型肽Ile-Arg-Met-Lys-Ile-Gly-Val-Met-Phe-Gly-Asn-Pro-Glu-Thr-Thr-Thr-Gly-Gly-Asn-Ala-Leu-Lys-Phe-Tyr，并研究了其与一些ssDNA和ssRNA的相互作用。圆二色光谱表明，添加ssDNA dACCTAGTC引起的肽构象变化比添加ssRNA rACCUAGUC引起的变化快得多。添加中性DNA或2'-5'连接的寡核苷酸不会导致肽的构象变化。