小球藻染色体的分子特征：弯曲DNA的筛选
Molecular characterization of Chlorella chromosomes: screening of bent DNAs.
作者信息
Noutoshi Y, Maki S, Higashiyama T, Yamada T
机构信息
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Japan.
出版信息
Nucleic Acids Symp Ser. 1995(34):73-4.
As a model for plant-type chromosomes, we have been characterizing molecular organization of Chlorella vulgaris (C-169) chromosome I. To locate structurally important chromosome regions, bent DNA fragments of this chromosome were systematically obtained and analyzed. By two-dimensional polyacrylamide gel electrophoresis, 150 clones containing bent DNA fragments were screened from C. vulgaris genomic libraries. These clones were classified into 3 groups by Southern hybridization. A group that hybridized with all Chlorella chromosomes contained fragments carrying a retroposon-like element. A clone (p2DS-66A) derived from chromosome VI strongly hybridized with chromosome I as well as the others. The corresponding chromosome I sequences (pI-21 and pI-31) were analyzed in detail.
作为植物型染色体的模型，我们一直在对普通小球藻（C-169）一号染色体的分子组织进行表征。为了定位结构上重要的染色体区域，我们系统地获取并分析了该染色体的弯曲DNA片段。通过二维聚丙烯酰胺凝胶电泳，从普通小球藻基因组文库中筛选出了150个含有弯曲DNA片段的克隆。通过Southern杂交将这些克隆分为3组。与所有小球藻染色体都杂交的一组包含携带类反转录转座子元件的片段。一个源自六号染色体的克隆（p2DS-66A）与一号染色体以及其他染色体都强烈杂交。对相应的一号染色体序列（pI-21和pI-31）进行了详细分析。
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